show Abstracthide AbstractAge is one of the main risk factors for cancer development. The incidence of prostate cancer, as a multifactorial disease, also depends upon demographic factors, race, and genetic predisposition. Prostate cancer most frequently occurs in men over 60 years of age, indicating a clear association between age and disease onset. Carcinogenesis is followed by the deregulation of hundreds of genes, and some of these changes could serve as biomarkers for diagnosis, prognosis, prediction of drug therapy efficacy, as well as possible therapeutic targets. We performed bioinformatic analysis of TCGA data and RNA-Seq transcriptome profiling of a cohort of Russian patients to reveal prognostic markers of locally advanced lymph node-negative prostate cancer (LAPC). We also aimed to identify markers of the most common molecular subtype carrying the fusion transcript TMPRSS2-ERG.